Ethanol is a proven alternative energy source for oil in the transportation sector. Ethanol can be derived from cellulosic biomass material, including wheat and rice straw, switchgrass, paper pulp, agricultural waste products like corn cobs and corn stover, citrus, algae, and more. Currently, the cellulosic biofuel industry is in its infancy - the economic opportunities are tremendous but the scientific principles are nascent. Clearly the high cost of converting biomass to sugars is impeding the growth of this industry. Like a number of scientific discoveries in genetic engineering that helped propel the biotech industry in the 80s, some scientific breakthrough is much needed for the biofuel industry to take roots.
In a recent research article published in PNAS, a group of researchers demonstrated the use of an engineered strain of bacteria to help produce ethanol in high yields. Thermophilic bacterial strains, currently used in aiding the fermentation process of sugars in biomass, produce organic acids (such as acetic acid and lactic acid) in addition to ethanol. Using knockout gene technology, the current research group was able to engineer a stable bacterial strain (named ALK2) that can be used for 100% ethanol production. The derived bacterial phenotype was also stable for more than 150 generations in culture.
So is ALK2 the magic bullet for the use of biomass in ethanol production at an industrial scale?. The authors are cautious. They note that future experiments are needed to close the gap between the maximum concentration of ethanol produced and the maximum concentration tolerated using this strain but they seem optimistic.